Imaging Covid-19, spike protein and LNP’s – research - Choice Not Coercion Imaging Covid-19, spike protein and LNP’s

See the research below that has isolated and imaged the different aspects of this pandemic; from the virus itself, to the spike protein and the lipid nanoparticles:

mRNA-lipid nanoparticle COVID-19 vaccines: Structure and stability https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8032477/

New insights into the structure of Comirnaty Covid-19 vaccine: A theory on soft nanoparticles with mRNA-lipid supercoils stabilized by hydrogen bonds https://www.biorxiv.org/content/10.1101/2022.12.02.518611v1.full

Figure 1.

AFM height-contrast images (A-D) and corresponding 3D reconstructions (E-F) of Comirnaty vaccine and Doxil immobilized on glass surface. (A) and (E) show a freshly diluted Comirnaty sample representing the jab inoculated into the deltoid muscle; (B) and (F) show a 1-day old sample stored at 4°C, representing the unused leftover; (C) and (G) show a refrozen sample, representing unintended acceleration of fragmentation by refreezing the leftover vaccine; (D) and (H) show a freshly opened Doxil sample.

Electron cryotomography of SARS-CoV-2 virions reveals cylinder-shaped particles with a double layer RNP assembly https://www.nature.com/articles/s42003-022-04183-1

New Images of Novel Coronavirus SARS-CoV-2 Now Available https://www.niaid.nih.gov/news-events/novel-coronavirus-sarscov2-images

Snapshots from Cryo-ET of active SARS-CoV-2 virions https://www.biorxiv.org/content/10.1101/2023.10.10.561643v1.full

Pleomorphology of active virus particles. a) Central sections (7.5 nm thick) through the cryo-ET tomograms of the ancestral virions. b) Violin plot representation of the two longest diameters (L1 and L2) in 46 virus particles. c) Tomograms slices of three virions with very large diameters (Left: L1:139, L2:102; Middle: L1:125, L2:119; Right: L1:150, L2:125). d) Central slices of virions on carbon film.

Vaccine mRNA Can Be Detected in Blood at 15 Days Post-Vaccination https://www.mdpi.com/2227-9059/10/7/1538

Cutting Edge: Circulating Exosomes with COVID Spike Protein Are Induced by BNT162b2 (Pfizer–BioNTech) Vaccination prior to Development of Antibodies: A Novel Mechanism for Immune Activation by mRNA Vaccines https://journals.aai.org/jimmunol/article/207/10/2405/234284/Cutting-Edge-Circulating-Exosomes-with-COVID-Spike

A Case Report: Multifocal Necrotizing Encephalitis and Myocarditis after BNT162b2 mRNA Vaccination against COVID-19 https://www.mdpi.com/2076-393X/10/10/1651

Nuclear translocation of spike mRNA and protein is a novel pathogenic feature of SARS-CoV-2 https://pubmed.ncbi.nlm.nih.gov/36203551/

Autopsy-based histopathological characterization of myocarditis after anti-SARS-CoV-2-vaccination https://link.springer.com/article/10.1007/s00392-022-02129-5#Sec3

Deltoid:

A The jab site in the deltoid muscle reveals focal inflammation. The composition is similar to the phenotype of the myocardial infiltrates showing predominantly, B CD3 and C CD4-coexpressing lymphocytes and D interspersed CD68-positive macrophages

Heart:

A Inflammatory focus in the left ventricular wall of case 2. B The infiltrate is predominantly composed of CD68-positive macrophages and C CD3-positive T-lymphocytes with (D) co-expression of CD4

Intraventricular:

A Lymphocytic aggregates in the interventricular septum of case 1 with associated myocardiocyte destruction. B The infiltrate is predominantly composed of CD3-positive T-lymphocytes and C CD68-positive macrophages. D In lower magnification two foci of CD4-positive lymphocytes are evident (D)